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958 Publications visible to you, out of a total of 958
Three-dimensional reconstruction and quantification of cervical carcinoma invasion fronts from histological serial sections.
ProstataCA

Abstract (Expand)

The analysis of the three-dimensional (3-D) structure of tumoral invasion fronts of carcinoma of the uterine cervix is the prerequisite for understanding their architectural-functional relationship. … The variation range of the invasion patterns known so far reaches from a smooth tumor-host boundary surface to more diffusely spreading patterns, which all are supposed to have a different prognostic relevance. As a very decisive limitation of previous studies, all morphological assessments just could be done verbally referring to single histological sections. Therefore, the intention of this paper is to get an objective quantification of tumor invasion based on 3-D reconstructed tumoral tissue data. The image processing chain introduced here is capable to reconstruct selected parts of tumor invasion fronts from histological serial sections of remarkable extent (90-500 slices). While potentially gaining good accuracy and reasonably high resolution, microtome cutting of large serial sections especially may induce severe artifacts like distortions, folds, fissures or gaps. Starting from stacks of digitized transmitted light color images, an overall of three registration steps are the main parts of the presented algorithm. By this, we achieved the most detailed 3-D reconstruction of the invasion of solid tumors so far. Once reconstructed, the invasion front of the segmented tumor is quantified using discrete compactness.

Authors: U. D. Braumann, J. P. Kuska, J. Einenkel, L. C. Horn, M. Loffler, M. Hockel

Date Published: 19th Oct 2005

Publication Type: Not specified

Human Diseases: cervical cancer

SOM portrayal of the peripheral blood transcriptome
LIFE - Leipzig Research Center for Civilization Diseases

Abstract (Expand)

Background: The transcriptome of healthy blood offers an option to characterize the physiological state of an individual with diagnostic impact. As a prerequisite such application requires the understanding … of the variability of the expression landscape of healthy individual’s as a function of factors such as age, gender, and aspects of the human constitution and lifestyle. Previous studies mostly were limited to only small population sizes and thus lack representativeness in many respects. Methods and data: The present thesis provides an extensive and detailed study on the gene expression landscape of peripheral blood of healthy individuals based on transcriptome data of 3,388 individuals screened in the population study LIFE between 2011 and 2014. For analysis we applied a neural network technique using self-organizing maps (SOM). Our home-made R-program ‘oposSOM’ enables to reduce the dimension of expression data from tens of thousands of genes to a few thousand ‘meta-genes’ and generates portraits of transcriptional activity that allowed us the sample-to-sample comparison of expression patterns. Results: We disentangled the expression patterns of the portraits into 15 well separated modules of co-expressed genes. Their activation patterns allowed us to aggregate the participants into three types (‘1’, ‘M’, ‘2’). Enrichment techniques extracts the functional context of the modules and revealed that corresponding cellular processes are selectively activated and de-activated in a type-specific fashion: For example, ‘Inflammation’ and ‘Blood coagulation’ are activated in type ‘1’ and ‘Metabolic activity’ and ‘Translation’ in Type ‘2’ samples. We map clinical parameters (complete blood count, lifestyle status, medication and the anthropometric body and BMI type) into the expression landscape in order to study the association between them and the transcriptome landscape. We found that red blood cell components, drug consumption of several drug classes affecting the cardio-vascular system or blood forming organs, tobacco and alcohol consumption, and the BMI types of obese participants highly correlate with meta-genes in the type ‘1’ area, whereas type ‘2’ meta-genes maximal correlate with the lymphocyte count and pre-obese characteristics of the participants. For blood count parameters and medication we found a gender-specific bias. Conclusion: Our analysis provides a comprehensive description of the human blood transcriptome in terms of a series of characteristic expression modules and of health-relevant factors. It provides a healthy reference system for blood transcriptome profiling studies in healthcare to extract potential associations between emerging diseases and the gene expression patterns in clinical research.

Author: M. Schmidt

Date Published: 9th Jul 2005

Publication Type: Not specified

Assessing the integration of information system components
Management of health information systems

Abstract (Expand)

This paper presents formal approaches for assessing the integration of information system components. They were developed to support decisions in the strategic management of information systems. The … fulfillment of integration requirements, the dependency of information system components on each other, and the heterogeneity of the integration infrastructure are the major assessment criteria.The meta-model 3LGM2A is the (semi-)formal base for methods introduced here. The fulfillment of integration requirements is checked by matching sets of application components that have specific requirements (requirements domains) with sets of application components that exchange data or call operations related to the requirements (communication domains). Requirements categories support the handling of the numerous specific requirements.For assessing the complexity of an information system or its subsystems the figures degree of informational dependence, degree of functional dependence, and degree of heterogeneity are defined.

Authors: Thomas Wendt, Birgit Brigl, Alfred Winter

Date Published: 2005

Publication Type: InCollection

3LGM(2)-Modelling to Support Management of Health Information Systems
Management of health information systems

Abstract (Expand)

Both regional health information systems and hospital information systems need systematic information management. Due to their complexity information management needs a thorough description or model … of the managed HIS. The three layer graph based meta model (3LGM(2)) and the 3LGM(2) tool provide means for effectively modeling HIS. The 3LGM(2) tool has been used to build a model of the health information system of the German federal state Saxony. The model is not only used to support the further development of the Saxonian health information system but also for supporting strategic information management planning in the medical center of Leipzig University. Acceptance of the method depends strictly on its integration in management structures on the institutional, regional, national or even European level.

Authors: Alfred Winter, B. Brigl, G. Funkat, A. Haber, O. Heller, T. Wendt

Date Published: 2005

Publication Type: Journal article

Supporting Information Management for Regional Health Information Systems by Models with Communication Path Analysis
Management of health information systems

Abstract

Not specified

Authors: Alfred Winter, B. Brigl, O. Heller, Ulrike Mueller, Alexander Struebing, T. Wendt

Date Published: 2005

Publication Type: InCollection

Modellieren von Krankenhausinformationssystemen am Beispiel des 3LGM^2-Baukastens
Management of health information systems

Abstract

Not specified

Authors: Alfred Winter, Birgit Brigl, Thomas Wendt

Date Published: 2005

Publication Type: InCollection

Architectural Quality Criteria for Hospital Information Systems
Management of health information systems

Abstract

Not specified

Authors: Birgit Brigl, Gudrun Hübner-Bloder, Thomas Wendt, Reinhold Haux, Alfred Winter

Date Published: 2005

Publication Type: InCollection

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