Single cell expression portraits

Each cell's expression landscape is described by the metagene expression values. They are arranged according to the underlying SOM grid and visualized by an appropriate color gradient (red and blue colors indicate over- and underexpressed metagenes, respectively; green and intermediate colors indicate no or low differential expression). The color patterns emerge as smooth textures representing the fingerprint of transcriptional activity of each cell and are mutually comparable.

Spot module reports

Complementary approaches were applied to define expression modules using the metagene landscape. The reports comprise several visualizations and information of the modules obtained:
Group overexpression spot modules collect metagenes consistently overexpressed in the different groups, respectively.

k-means modules are defined by applying k-means clustering to the metagene expression data.